DEPOD the human DEPhOsphorylation Database is a manually curated database harboring human phosphatases, their experimentally verified protein and non protein substrates and the dephosphorylation site information. It also harbors information about the phosphorylating protein kinases, protein interaction partners and pathways involving these phosphatases and substrates. DEPOD aims to be a valuable resource for studying human phosphatases and their substrate specificities and molecular mechanisms; phosphatase targeted drug discovery and development; connecting phosphatases with kinases through their common substrates; completing the human phosphorylation/dephosphorylation network.