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Database: DynamProt

The Kahn Dynamic Proteomics project aims at monitoring the position and amounts of endogenous proteins in individual living human cells. This is based on the Library of Annotated Reporter Cell-clones (LARC). Each cell-line clone contains an endogenous protein fused to yellow fluorescent protein (YFP), expressed from its endogenous chromosomal location with its natural regulation. Labeling with YFP was done by exon-tagging (also known as CD-tagging), where YFP, flanked by splice signals, was delivered into the genome using a retrovirus. YFP is then spliced into the protein as a new exon. Protein identity was established by sequencing the cDNA. The cell line used is the H1299 non-small lung cell carcinoma line which stably expressed the viral receptor. This is a robust cell-line with a nicely spread cell morphology that is highly photogenic for microscopy purposes. Several lines of evidence indicate that over 2/3 of the tagged proteins retain their proper functionality and localization.

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