As one of the most important and ubiquitous post-translational modifications (PTMs) in plants, the reversible phosphorylation catalyzed by protein kinases is involved in regulating a wide range of biological processes such as cellular metabolism, signal transduction and environmental response (Ranjeva et al., 1987; Mundy et al., 2002; Kusakina et al., 2012). Recently with the development of phosphopeptide enrichment techniques and high-throughput mass spectrometry (HTP-MS) technology, large scale phosphoproteomics data in plants have been emerged at a quick pace, stimulating the creation of multiple phosphorylation databases to store and organize them. However, a more comprehensive phosphorylation database of plants is still necessary and useful. In this work, we develop a database of dbPPT 1.0 (database of Phospho-sites in PlanTs), which contains large-scale phosphorylation sites (p-sites) based on mass spectrometry across 20 plant species. Most of the datasets in dbPPT 1.0 were manually curated from literature published before July, 2014.