Here we present the improved version of the ChiTaRS database (ChiTaRS 2.1) of 20,750+6,224+2,151+4+4+8+13+5 chimeric transcripts in humans, mice, fruit flies, zebrafishes, cows, rats, pig and yeast. In the current version we extended the experimental data evidence as well as included a novel type of the sense antisense chimeric transcripts of the same gene confirmed experimentally by RT PCR, qPCR, RNA sequencing and mass spec peptides.