Since our publication of TSGene 1.0 in 2013, we have received many inquires for more details about our TSGene. Addtionally, many more cancer datasets, especially those from pan-cancer studies, have been released since we published TSGene. In this updated version, we collected hundreds of additional tumor suppressor genes from literature. TSGene2.0 now contains 1217 human genes (1018 coding and 199 non-coding genes) curated from a total of over 9000 PubMed abstracts.