DEPOD - the human DEPhOsphorylation Database is a manually curated database harboring human phosphatases, their experimentally verified protein and non-protein substrates and the dephosphorylation site information. It also harbors information about the phosphorylating protein kinases, protein interaction partners and pathways involving these phosphatases and substrates. DEPOD aims to be a valuable resource for studying human phosphatases and their substrate specificities and molecular mechanisms; phosphatase-targeted drug discovery and development; connecting phosphatases with kinases through their common substrates; completing the human phosphorylation/dephosphorylation network.