Increasing evidence reveals that diverse non-coding RNAs (ncRNAs) play critically important roles in viral infection. Viruses can use diverse ncRNAs to manipulate both cellular and viral gene expression to establish a host environment conducive to the completion of the viral life cycle. Hence, we have updated the ViRBase v2.0 database (www.rna-society.org/virbase/) by integrating experimental and prediction interactions from manual literature curation and other resources under one common framework. The new developments in ViRBase v2.0 include (i) an nearly 70-fold increase in ncRNA-associated virus–virus, virus–host, host–virus and host–host interactions enhancement compared with the previous version; (ii) integrating experimental and prediction evidence from 5 resources and prediction algorithms for each interaction; (iii) providing a confidence score for each interaction; and (iv) an increase of species coverage to 119.